High-Glucose DMEM Powder with Phenol Red, 100 L

This high-glucose DMEM powder is designed for laboratories and production-support teams that require a dry, scalable format for routine mammalian cell culture medium preparation. The formulation includes phenol red as a pH indicator, 4.5 g/L D-glucose for energy metabolism, 4 mM L-glutamine, and 0.11 g/L sodium pyruvate. It does not contain sodium bicarbonate or HEPES, so the user should add the appropriate buffer system during preparation to match incubator settings, closed-vessel conditions, and internal SOPs.

Key release specifications include pH 6.0-6.4 and osmolality 240-280 mOsmol/kg, with endotoxin specified at ≤1 EU/mL. The supplied 100 L powder format supports efficient stocking, batch preparation, and reduced liquid storage volume for procurement teams managing multi-site demand. For related formulations and alternatives, see the classical media category.

• Preparation of high-glucose DMEM for fibroblasts, smooth muscle cells, and comparable adherent mammalian cell culture models.
• Routine scientific and basic research workflows using 37 °C incubation with 5-10% CO2, after appropriate buffering and final medium adjustment.
• Complete medium preparation with serum supplementation where required; the source recommendation is 10% FBS, while the powder itself is animal origin-free as supplied.
• R&D scale-up studies where a dry powder medium provides consistent formulation control and simplified inventory management.
• Process development groups comparing glucose-rich DMEM conditions with or without added bicarbonate or HEPES, depending on vessel type and gas-exchange profile.

Store the unopened powder at 2-8 °C protected from light. The stated shelf life is 48 months when stored under recommended conditions. Shipments are arranged on blue ice according to the listed shipping condition, with free worldwide shipping from CellCultureMedia. During reconstitution, use qualified water, validated mixing procedures, and appropriate sterile filtration or aseptic processing according to your facility requirements. Because sodium bicarbonate and HEPES are not included, confirm final pH, osmolality, and buffer concentration before use.

CellCultureMedia supplies CCM-CM-0039 for research and bioprocessing customers that need specification-controlled raw materials and procurement-ready documentation. Manufacturing and release controls are aligned with ISO-style quality management expectations, and a Certificate of Analysis can be provided on request for lot-specific review of parameters such as pH, osmolality, endotoxin, appearance, and formulation identity. For documentation expectations, visit quality and documentation, or request a quote for bulk pricing, standing supply, or multi-product sourcing.