Ham's F-12K Nutrient Medium, 500 mL

CCM-CM-0022 is a ready-to-use liquid Ham's F-12K nutrient medium, also known as the Kaighn modification, designed for cell culture environments requiring defined inorganic salts, amino acids, vitamins, glucose, phenol red, and L-glutamine in a balanced classical formulation. The medium is supplied with 1.26 g/L D-glucose and 2 mM L-glutamine, supporting routine growth and maintenance studies where a complete medium is prepared by adding serum or other laboratory-defined supplements. It is suitable for organizations standardizing classical media across R&D, academic, and bioprocess development laboratories.

The product is manufactured as an animal-origin-free medium as supplied, with no animal-derived components intentionally included in the base formulation. Source specifications indicate use with human hepatocyte workflows under 37°C incubation and 5-10% CO2, typically after supplementation with 10% fetal bovine serum. Key release parameters include pH 7.5-7.9, osmolality 310-350 mOsmol/kg, filtration-based sterility processing, endotoxin not more than 1 EU/mL, and a 12-month shelf life when stored as directed. These attributes support lot-to-lot review, incoming material qualification, and controlled inventory planning for procurement and process teams.

• Research culture of human hepatocytes and related liver-cell model systems using F-12K-compatible protocols.
• Preparation of complete medium by adding 10% fetal bovine serum or other validated supplements required by the laboratory workflow.
• Routine expansion, maintenance, and experimental conditioning of adherent cells requiring phenol red pH indication and 2 mM L-glutamine.
• Comparative media screening where pH, osmolality, glucose level, and endotoxin specification must be documented before use.
• Academic, biotech, and biopharma R&D programs that need a 500 mL liquid format for controlled small-batch use.

For multi-bottle orders, recurring supply, or alternative packaging requirements, teams can request a quote with target quantities, delivery region, and any documentation needs. CellCultureMedia can support procurement reviews that compare formulation, storage requirements, and shelf-life constraints across compatible media.

Store CCM-CM-0022 at 2-8°C and protect the bottle from light to maintain phenol red performance and formulation stability through the stated 12-month shelf life. The product ships on blue ice and should be transferred to refrigerated storage immediately on receipt. Do not freeze unless a validated internal procedure permits it, and minimize repeated warming cycles during use. Aseptic technique is required after opening; dispense only in a clean cell culture workspace, keep containers closed when not in use, and record opening dates according to your site inventory procedure.

CellCultureMedia supplies this medium through ISO-aligned manufacturing and release workflows focused on sterility, endotoxin control, physicochemical testing, and traceable lot documentation. A Certificate of Analysis is available on request for quality review, including applicable specifications for pH, osmolality, endotoxin, sterility processing, and shelf life. For vendor qualification, audit support, or documentation planning, visit our quality information page. Free worldwide shipping is available for qualified orders, with temperature-controlled dispatch selected to match the blue-ice shipping condition.