Glucose-Free DMEM Medium with Phenol Red

CCM-CM-0010 is a glucose-free DMEM medium for laboratories that need a defined classical medium base while controlling external carbon source addition. The formulation is liquid, sterile-filtered, and specified as animal-origin-free before user supplementation. It contains phenol red for visual pH indication and 4 mM L-glutamine as a nitrogen source. It does not contain D-glucose, sodium pyruvate, or HEPES, making it suitable for experiments where these components must be introduced separately, omitted, or varied by the user.

This medium is designed for culture systems such as fibroblasts, smooth muscle cells, and related adherent cell models when supplemented appropriately, typically with 10% fetal bovine serum according to the stated formulation guidance. Standard incubation conditions are 37°C with 5–10% CO2. Key release specifications include pH 7.0–7.4, osmolality 300–340 mOsmol/kg, endotoxin ≤1 EU/mL, sterile filtration, and a 12-month shelf life when stored as directed. For adjacent formulations and comparable classical media formats, see the classical media category.

• Glucose-controlled cell culture studies where investigators add a defined concentration of D-glucose or alternative carbon source after medium preparation.
• Culture of fibroblasts, smooth muscle cells, and other compatible adherent cell types using validated serum supplementation, commonly 10% fetal bovine serum.
• Basic research workflows requiring DMEM chemistry with phenol red monitoring but without sodium pyruvate or HEPES buffering.
• Process development, media comparison, and formulation screening where pH, osmolality, endotoxin, and sterility specifications must be documented for procurement review.
• Laboratory standardization projects in which a single glucose-free medium base is aliquoted or supplemented under controlled internal procedures.

Store CCM-CM-0010 at 2–8°C protected from light. The product is supplied as a ready-to-use liquid medium but should be equilibrated and supplemented according to the validated protocol for the target cell type before use. Do not freeze the bottle unless an internal stability study supports that practice. Use aseptic technique when opening, minimize repeated warming cycles, and keep the container closed when not dispensing. Shipping is performed on blue ice to support refrigerated transit, and receiving teams should inspect temperature condition, bottle integrity, lot identification, and expiry date before release into laboratory inventory.

CellCultureMedia supplies CCM-CM-0010 for research and bioprocessing organizations that require consistent documentation, lot traceability, and procurement-ready specifications. Manufacturing and quality release are aligned with ISO-style quality management principles, including sterile filtration controls and specification review for pH, osmolality, endotoxin, shelf life, and storage requirements. A Certificate of Analysis is available on request through the quality documentation page. For multi-bottle requirements, standing supply, or institution-level purchasing, request pricing and availability through request a quote. Free worldwide shipping is offered where cold-chain service is available.