DMEM/F-12 (1:1) Medium with Phenol Red and L-Glutamine

CCM-CM-0018 is a ready-to-use liquid DMEM/F-12 (1:1) medium formulated for mammalian cell culture workflows that require a balanced mixture of amino acids, vitamins, salts, glucose, and trace components. The medium includes phenol red, 3.15 g/L D-glucose, and 2.5 mM L-glutamine, and it is supplied without HEPES for users who prefer bicarbonate-buffered culture in a controlled CO2 incubator. The recommended culture environment is 37 °C with 5–10% CO2, using appropriate aseptic technique and vessel geometry for the cell system.

This product is suitable for teams sourcing classical media for neuronal, glial, endothelial, and related adherent or suspension culture models. The base formulation is classified as animal-origin-free before supplementation; many workflows add 10% fetal bovine serum or other qualified supplements according to internal protocols. Release specifications include pH 7.0–7.4, osmolality 290–330 mOsmol/kg, endotoxin ≤1 EU/mL, and filter sterilization. The phenol red indicator provides a practical visual check for pH drift during routine handling, while final acceptance should follow laboratory quality procedures.

• Maintenance and expansion of neuronal, glial, endothelial, and other mammalian cell types that are validated by the user for DMEM/F-12 (1:1) chemistry.
• Serum-containing culture systems requiring addition of 10% fetal bovine serum, qualified serum alternatives, growth supplements, or project-specific additives.
• Comparative medium screening where a non-HEPES formulation is preferred to match CO2-controlled incubator conditions.
• Academic, biotechnology, and biopharma R&D workflows requiring defined purchasing specifications for pH, osmolality, endotoxin, sterility, and shelf life.
• Scale-up planning and small-batch process development where consistent 500 mL liquid media packaging supports standardized documentation and inventory control.

For recurring usage, multi-unit demand, or scheduled replenishment, procurement teams can submit requirements through request a quote. Please include annual volume, delivery region, required documentation, and any receiving constraints so CellCultureMedia can align pricing, packaging, and dispatch planning with your internal purchasing process.

Store CCM-CM-0018 at 2–8 °C protected from light. Do not use the medium after the stated 12-month shelf life or after internal hold-time limits have been exceeded following opening. The product ships on blue ice to support refrigerated transit, and containers should be inspected on receipt for closure integrity, appearance, and temperature exposure according to site procedures. Warm only the required volume before use, avoid repeated temperature cycling, and handle under aseptic conditions in a qualified biosafety cabinet or equivalent controlled environment.

CellCultureMedia supplies CCM-CM-0018 for research and bioprocessing use with specification-focused documentation for procurement and technical review. Manufacturing and release controls are ISO-aligned and include filter sterilization, endotoxin testing, pH testing, osmolality testing, and visual inspection. A lot-specific Certificate of Analysis is available on request, and additional quality information can be reviewed through our quality documentation resources. The 500 mL pack is listed at $48.61, with free worldwide shipping available for standard eligible orders. For institutional purchasing, consolidated ordering and bulk pricing can be coordinated to reduce administrative workload and support lot planning.