DMEM/F-12 1:1 Medium with L-Alanyl-Glutamine

CCM-CM-0020 is a ready-to-use liquid DMEM/F-12 1:1 medium for laboratories requiring a balanced nutrient platform with the buffering, amino acid, vitamin, salt, glucose, and trace component profile commonly selected for complex mammalian cell culture. The formulation includes phenol red as a visual pH indicator and L-alanyl-glutamine as a stabilized glutamine source designed to improve handling consistency compared with less stable glutamine supplementation. HEPES is not included, allowing users to manage buffering through incubator carbon dioxide settings and their own process requirements.

The medium is specified for culture at 37 °C in a 5–10% CO2 incubator environment and is commonly paired with 10% fetal bovine serum where serum-supported growth is required. The supplied base medium is classified as animal-origin-free before user-added supplements. Key release specifications include pH 7.0–7.4, osmolality 290–330 mOsmol/kg, endotoxin ≤1 EU/mL, sterile filtration, and a 12-month shelf life when stored as directed. This product belongs to the classical media portfolio for research and foundational cell culture work.

• Routine culture and expansion of neuronal, glial, endothelial, and other mammalian cell types where a DMEM/F-12 1:1 nutrient balance is appropriate.
• R&D assay preparation requiring a phenol-red-containing medium with visible pH indication during incubation, media exchange, and process observation.
• Serum-supplemented culture systems using 10% fetal bovine serum or laboratory-defined alternatives after internal qualification.
• Process development studies that require a liquid, sterile-filtered classical medium with documented pH, osmolality, endotoxin, shelf-life, and storage specifications.
• Comparability studies, media screening, and scale-up feasibility work where the absence of HEPES supports evaluation under CO2-controlled buffering conditions.

Store CCM-CM-0020 at 2–8 °C protected from light. Do not freeze unless your internal protocol has qualified freeze-thaw impact on pH, osmolality, and cell performance. Inspect the bottle before use for seal integrity, clarity, color shift, and particulate matter. Warm only the volume required for immediate work and use aseptic technique during all transfers. Because the medium does not contain HEPES, maintain validated CO2 control during incubation. The product ships on blue ice to support temperature management in transit; upon receipt, move bottles promptly to refrigerated storage and record lot information for traceability.

CellCultureMedia supplies CCM-CM-0020 for research, development, and bioprocessing environments that require consistent lot documentation and procurement-ready specifications. Manufacturing and release controls are aligned with ISO-style quality practices, including sterile filtration and lot-level checks for pH, osmolality, endotoxin, appearance, and shelf-life assignment. A Certificate of Analysis is available on request through our quality documentation process. The 500 mL pack is priced at $97.22, with free worldwide shipping for eligible orders. For standing orders, multi-bottle requirements, or matched-lot planning, submit requirements through request a quote so our team can confirm availability, lead time, and bulk pricing.