Complete Amniotic Fluid Cell Culture Medium

Complete Amniotic Fluid Cell Culture Medium is an animal-origin, ready-to-use medium for the expansion and maintenance of amniotic fluid-derived cells in scientific and basic research workflows. It is supplied under CellCultureMedia SKU CCM-CM-0043 in a 100 mL liquid pack. The medium is classified as a complete culture medium because serum and key supplements are already included; users should not plan routine serum addition unless their internal protocol has been specifically qualified for that change.

The formulation includes D-glucose for energy metabolism, L-glutamine as a nitrogen and carbon source, sodium bicarbonate for CO2-buffered pH control, fetal bovine serum as a complex growth-support component, and gentamicin for antibacterial support in controlled culture workflows. Product specifications include pH 7.0–7.6, osmolality 280–340 mOsmol/kg, endotoxin not more than 3 EU/mL, and filter sterilization. For related liquid media formats, see the classical media category.

• Culture of amniotic fluid-derived cells for academic, biotech, and contract research laboratory workflows.
• Routine cell expansion at 37°C in a humidified incubator with 4–6% CO2.
• Research protocols requiring a complete medium with pre-added serum, glutamine, bicarbonate buffer system, glucose, and gentamicin.
• Process evaluation where a defined incoming material profile is required for pH, osmolality, endotoxin, sterility method, storage condition, and shelf-life review.
• Small-volume qualification before larger procurement; the 100 mL format is suitable for feasibility work, method bridging, and controlled comparison against internally approved media.

Store CCM-CM-0043 frozen at -30°C to -5°C and protected from light. The stated shelf life is 18 months when the product remains within the specified storage range and original container integrity is maintained. Shipment is arranged on dry ice to preserve the frozen state during transit. On receipt, inspect the shipper, confirm that dry ice remains or that the product is still frozen, record the arrival condition, and transfer immediately to qualified frozen storage. Thaw only the volume needed for scheduled work, mix gently after thawing, and avoid repeated freeze-thaw cycles where possible because repeated thermal stress can affect serum-containing complete media. Use aseptic technique when opening and dispensing, and do not use if the container is damaged, visibly contaminated, or outside an approved receiving temperature excursion assessment.

CellCultureMedia supplies this medium for research and bioprocessing use with procurement-ready specifications, including liquid form, animal-origin classification, filter-sterilized status, endotoxin limit, pH range, osmolality range, shelf life, storage condition, and dry-ice shipping requirement. Manufacturing and release controls are managed under ISO-aligned quality practices, with lot traceability and documentation support available for purchasing, R&D, and process teams. A Certificate of Analysis can be requested during ordering or through our quality documentation channel. For price confirmation, bulk demand, standing orders, or country-specific logistics, submit details through request a quote. The listed 100 mL price is USD 402.78, and eligible orders include free worldwide shipping subject to destination and cold-chain feasibility.