Xeno-Free Lymphocyte Serum-Free Medium with HSA

CCM-SC-0010 is designed for organizations that need a defined lymphocyte culture medium without serum or animal-derived components. The formulation is xeno-free and includes human serum albumin as a protein supplement, supporting controlled media qualification for R&D, assay development, and scale-up planning. Phenol red is included for visual pH indication during routine handling, while D-glucose provides an energy source for actively cultured cells.

The glutamine system combines 1.5 mM L-glutamine with 1.25 mM L-alanyl-L-glutamine to provide both an immediately available amino acid source and a more stable dipeptide form. The product is supplied as a liquid medium in a 500 mL format with sterile filtration, endotoxin specification of ≤3 EU/mL, pH 7.0–7.4, and osmolality of 280–330 mOsmol/kg. For related defined formulations, visit our stem cell media category.

• Lymphocyte culture under serum-free and xeno-free conditions for research, development, and process evaluation.
• Media comparison studies where defined protein supplementation, phenol red, and controlled glutamine chemistry are required.
• Cell and gene research workflows that require documentation of serum level, animal-origin status, pH, osmolality, endotoxin, and sterility specifications.
• Small-batch process development, cell expansion feasibility work, and upstream workflow optimization at 37 °C with 5–10% CO2.
• Procurement standardization for laboratories seeking a 500 mL liquid medium format with a 12-month shelf life and lot-level quality records.

Store CCM-SC-0010 at 2–8 °C and protect from light. The product ships on blue ice to maintain refrigerated transport conditions. Handle using aseptic technique after opening, keep the bottle closed when not in use, and minimize repeated warming cycles during routine work. The specified shelf life is 12 months when the unopened product is stored under recommended conditions. Before use, equilibrate only the required working volume to the intended culture temperature and CO2 environment.

CellCultureMedia supplies CCM-SC-0010 for research and bioprocessing customers that require traceable specifications and procurement-ready documentation. Manufacturing and release controls are ISO-aligned, with sterility, endotoxin, pH, osmolality, formulation attributes, storage conditions, and shelf-life information available for quality review. A Certificate of Analysis can be requested through our quality documentation page or during purchasing. For volume requirements, standing orders, or multi-site supply planning, use the request quote form; qualified orders are eligible for free worldwide shipping.