Xeno-Free hMSC Expansion Medium With Phenol Red

CCM-SC-0013 is a liquid expansion medium specified for hMSC culture where animal-origin risk reduction, lot documentation, and reproducible handling are procurement priorities. The formulation is designated serum-free and xeno-free, and it includes phenol red for visible pH monitoring during routine culture handling. The additive profile also includes a non-serum replacement component listed as an FBS substitute, supporting workflows that require reduced reliance on conventional serum-containing systems.

This 500 mL format is intended for R&D laboratories, process development groups, and pilot-scale cell culture teams evaluating human mesenchymal stem cell expansion conditions. The medium is sterile-filtered, supplied frozen, and specified for use under 37 °C and 5–10% CO2 incubation. Release specifications include pH 7.0–7.4 and osmolality 270–340 mOsmol/kg, providing a defined operating window for incoming quality review, media comparison studies, and controlled batch records. For related formats and complementary reagents, review our stem cell media category.

• Expansion of human mesenchymal stem cells in serum-free, xeno-free culture systems for research, development, and manufacturing science programs.
• Media screening, process characterization, and scale-up evaluation where pH, osmolality, endotoxin, sterility, and shelf-life specifications must be documented before use.
• Incubator workflows operated at 37 °C with 5–10% CO2, including flask, multiwell, and closed or semi-closed vessel assessments when validated by the user.
• Comparability studies involving serum-reduced or serum-free transition work, including evaluation of morphology, attachment, growth rate, harvest timing, and downstream assay compatibility.
• Procurement-standardized supply for academic cores, biotechnology laboratories, and contract development environments requiring consistent 500 mL frozen liquid media lots.

Store CCM-SC-0013 frozen at -30 to -5 °C and protected from light. The product is shipped on dry ice to maintain frozen condition during transit; inspect the container on receipt and transfer promptly to qualified frozen storage. Shelf life is 12 months when stored as specified and unopened. Thaw using a controlled laboratory procedure, mix gently after thawing, and avoid repeated freeze-thaw cycles unless the workflow has been qualified internally. Use aseptic technique after opening, and discard material that shows visible contamination, unexpected color shift, compromised packaging, or storage excursion outside the approved range.

Each lot is manufactured under ISO-aligned quality practices and released against defined specifications, including sterile filtration, endotoxin ≤3 EU/mL, pH 7.0–7.4, and osmolality 270–340 mOsmol/kg. A Certificate of Analysis can be requested for lot review, vendor qualification, receiving inspection, or internal change-control documentation. Additional quality information is available through our quality and documentation resources.

The listed pack size is 500 mL with a unit price of $1,254.17. Free worldwide shipping is available, with dry-ice logistics used for frozen delivery. For standing orders, multisite supply, or bulk purchasing, submit requirements through request a quote so CellCultureMedia can confirm current availability, shipping lane feasibility, and volume pricing.