Vitamin A-Free Serum-Free Supplement 50X

This vitamin A-free serum-free supplement is a concentrated 50X liquid additive for defined cell culture media. It is designed for workflows where the base medium is supplemented at 1X final concentration and where vitamin A, retinol, or retinoid contribution from the supplement must be excluded. The product is supplied under CCM-RG-0067 in a 10 mL bottle and is categorized within cell culture reagents for serum-free culture system design, media optimization, and experimental control.

Key release specifications include liquid form, animal-origin classification, sterile filtration, 24-month shelf life, and endotoxin not more than 20 EU/mL at 1X use concentration. The vitamin A-free formulation supports experiments that require tighter control over differentiation cues, oxidative environment, and supplement-driven variability. It is appropriate for procurement teams consolidating defined media components and for scientists comparing additive lots, serum-free formulations, or reduced-serum conditions in controlled culture systems.

• Serum-free culture of neuronal and other sensitive mammalian cell models where a defined 50X supplement is required.
• Retinoid-controlled studies that need a supplement specified as vitamin A-free to reduce background contribution from retinol-related components.
• Media development, component screening, and formulation comparison in R&D laboratories and process-development groups.
• Advanced cell culture manufacturing research where endotoxin-controlled, sterile-filtered additives are needed for reproducible batch preparation.
• Academic and biopharma workflows requiring a small-format 10 mL reagent for feasibility studies, pilot runs, or lot qualification before larger purchasing.

Store CCM-RG-0067 at -30 to -5 °C and protect it from light. The product ships on dry ice to maintain frozen conditions during transit. For best practice, thaw only under controlled laboratory conditions, mix gently without foaming, and avoid repeated freeze-thaw cycles by preparing single-use aliquots where appropriate. Maintain aseptic technique after opening because the supplement is supplied sterile-filtered, not terminally sterilized in the final user vessel. When stored unopened under the stated conditions, the assigned shelf life is 24 months from manufacture or as shown on the product label and lot documentation.

CellCultureMedia supplies this supplement through ISO-aligned sourcing, lot traceability, and specification-based release review. Available quality attributes include sterile filtration status, animal-origin classification, vitamin A-free designation, and endotoxin limit of ≤20 EU/mL at 1X. A Certificate of Analysis can be requested through quality documentation support, and procurement teams can use the CoA for incoming inspection, internal material qualification, and lot-to-lot comparison. For price confirmation, quantity breaks, and shipment scheduling, submit a request through request quote. Free worldwide shipping is available on eligible orders, with dry-ice packaging applied for frozen transport.