Serum-Free hMSC Medium, Phenol Red-Free

CCM-SC-0016 is a ready-to-use liquid medium for human mesenchymal stem cells, designed for serum-free culture systems that require no added serum. The medium is manufactured without phenol red and without fetal bovine serum substitute, supporting workflows where color-indicator-free medium and reduced undefined components are preferred. It is classified as animal-origin-free and is suitable for research, development, process evaluation, and cell manufacturing model work involving hMSC cultures.

The formulation contains 3.9 g/L D-glucose for carbohydrate supply, L-glutamine as a nitrogen and energy-related component, and sodium bicarbonate for buffering in a humidified CO2 incubator. Recommended culture conditions are 37℃ with 4-6% CO2. Key release specifications include pH 7.0-7.4, osmolality 280-320 mOsmol/kg, endotoxin ≤3 EU/mL, and sterility by filtration. For related defined media options, see the /categories/stem-cell-media/ catalog or contact CellCultureMedia for cross-referencing by cell type, bottle size, and documentation needs.

• Serum-free expansion and maintenance studies using human mesenchymal stem cells in R&D and process development laboratories.
• Phenol red-free culture systems where visual color indicators may interfere with analytical readouts, imaging, or assay background.
• Animal-origin-free medium selection for controlled raw-material strategies and defined component mapping.
• Small-scale screening, flask-based culture, and scale-down process models operated at 37℃ and 4-6% CO2.
• Procurement programs requiring documented pH, osmolality, endotoxin, sterility, shelf-life, storage, and shipping specifications for incoming material review.

Store CCM-SC-0016 at 2-8℃ and protect from light. The stated shelf life is 12 months when unopened and maintained under the specified storage conditions. Shipments are dispatched on blue ice to support refrigerated transit. Do not freeze unless a validated internal procedure has been established by the receiving laboratory. Before use, inspect the bottle for integrity, clarity, and lot labeling; equilibrate only the required working volume to the intended culture temperature. Use aseptic technique after opening and follow your site-specific hold-time rules for opened liquid media.

CellCultureMedia supplies CCM-SC-0016 for B2B research and bioprocessing customers that require consistent lot documentation and procurement-ready product data. Manufacturing and quality release are managed under ISO-aligned controls, with filtered sterility, endotoxin, pH, and osmolality specifications available for review. A Certificate of Analysis can be requested through /quality/ or during purchasing. Free worldwide shipping is available for this item, and our team can support lot reservation, standing orders, and quotation workflows through /request-quote/.