Serum-Free Hematopoietic Stem Cell Medium

CCM-SC-0025 is a complete serum-free medium designed for hematopoietic stem cell culture under controlled incubator conditions of 37 °C and 4–6% CO2. The formulation is supplied in liquid format and is intended for organizations requiring a defined medium for stem cell research, cell processing development, screening, and scale-up evaluation. Because no serum addition is required, procurement and process teams can reduce lot-to-lot variation associated with serum-containing systems while maintaining a simplified preparation workflow.

The medium includes D-glucose as an energy source, L-glutamine for nitrogen metabolism and biosynthetic support, and phenol red for visual pH monitoring during routine culture operations. Key release specifications include pH 7.0–7.4, osmolality 280–330 mOsmol/kg, endotoxin not more than 1 EU/mL, and filter-sterilized processing. For related defined media, review the stem cell media category or contact CellCultureMedia for pack-size planning and sourcing support.

• Culture of hematopoietic stem cells in serum-free, xeno-free research workflows where defined inputs are required.
• Process development for cell and gene manufacturing models, including media comparison, feeding strategy evaluation, and culture condition optimization.
• Academic and industrial R&D programs requiring a ready-to-use liquid medium with documented pH, osmolality, sterility, and endotoxin specifications.
• Small-scale expansion studies in flasks, plates, or closed-compatible vessels where 37 °C incubation and 4–6% CO2 conditions are used.
• Procurement standardization for laboratories moving away from serum supplementation and toward controlled, specification-based raw material selection.

Store CCM-SC-0025 at 2–8 °C protected from light. The product is shipped on blue ice to help maintain refrigerated transit conditions and has a stated shelf life of 12 months when stored as recommended and kept unopened. Use aseptic technique when opening and dispensing. Do not leave the bottle at room temperature longer than needed for preparation, and avoid repeated warming cycles. Confirm that the medium is clear and free of visible contamination before use; phenol red color may be used as a general visual indicator but should not replace pH verification for controlled studies.

CellCultureMedia supplies CCM-SC-0025 for research and bioprocessing environments that require documented raw material attributes. Production is managed under ISO-aligned quality practices, and lot-specific documentation can be requested through our quality documentation process. Available records may include Certificate of Analysis details covering sterility, endotoxin, pH, osmolality, and product identity. For standing orders, bulk volumes, or annual forecasts, submit a request through request a quote. Free worldwide shipping is available, with refrigerated packaging selected according to product handling requirements and destination.