Serum-Free Cell Culture Supplement 50X

CCM-RG-0066 is a concentrated 50X serum-free supplement for cell culture media optimization, supplied as a liquid 10 mL reagent. It is designed to be diluted into a compatible basal formulation or user-qualified medium system to support defined culture conditions without the routine use of whole serum. The product is classified as animal-origin and is filter-sterilized for aseptic laboratory and process-development handling. For teams sourcing defined additives across multiple programs, this item fits within CellCultureMedia’s cell culture reagents portfolio.

The supplement is used where a standardized, concentrated additive format is preferred for media preparation, small-batch screening, and scale-up studies. At the working 1X concentration, the specified endotoxin limit is ≤20 EU/mL. The stated shelf life is 24 months when stored under the recommended frozen, light-protected conditions. Because this is a supplement rather than a complete medium, users should qualify the final medium composition, dilution ratio, feeding schedule, and compatibility with their selected cell type and process parameters before routine adoption.

• Serum-free supplementation of compatible neuronal, primary-cell, or specialized mammalian cell culture systems during R&D media evaluation.
• Defined media development where procurement teams require a concentrated additive with documented sterility method, endotoxin specification, shelf life, and cold-chain shipping conditions.
• Cell and gene research and manufacturing-support workflows requiring controlled supplement addition during upstream process development, analytical model setup, or comparability studies.
• Small-scale formulation screening in plates, flasks, spinner systems, or other lab-scale vessels, followed by user qualification for larger preparation volumes.
• Replacement or reduction of undefined serum components in applications where researchers have already established that a 50X serum-free supplement is appropriate for the cell type and readout.

Store CCM-RG-0066 at -30 to -5 °C and protect the bottle from light. The product ships on dry ice to maintain the frozen cold chain during transit. Upon receipt, inspect the package condition, confirm the material remains frozen or suitably cold, and transfer it promptly to validated frozen storage. Thaw using a controlled, user-qualified procedure, mix gently to ensure homogeneity, and avoid unnecessary warm exposure. For repeated use, aliquoting under aseptic conditions may help reduce freeze-thaw cycling. Do not use material beyond the assigned expiry date or if container integrity has been compromised.

CellCultureMedia supplies CCM-RG-0066 for research and bioprocessing organizations that need clear specifications for purchasing, receiving, and quality review. Manufacturing and release controls are ISO-aligned, and the product is filter-sterilized with an endotoxin specification of ≤20 EU/mL at 1X. Lot documentation, including a Certificate of Analysis when available, can be requested through the quality documentation team. Current price for the 10 mL size is $563.89, with free worldwide shipping. For scheduled demand, multi-unit orders, or purchasing-system setup, submit a request through request a quote for lead time, availability, and bulk pricing confirmation.