Serum-Free hMSC Culture Medium, 500 mL

CCM-SC-0019 is a serum-free liquid medium intended for human mesenchymal stromal cell culture in research, process development, and advanced cell manufacturing environments. The formulation is supplied as a complete basal-format liquid medium that does not require serum addition and is classified as animal-origin-free. It contains D-glucose as an energy source, L-glutamine to support nitrogen metabolism, and sodium bicarbonate for CO2-buffered culture systems. The medium is designed for incubation at 37°C with 4–6% CO2, supporting standard hMSC culture conditions used across R&D and scale-up laboratories.

Key release specifications support procurement and QA review: pH 7.0–7.4, osmolality 280–320 mOsmol/kg, endotoxin ≤3 EU/mL, 12-month shelf life, and sterility by filtration. The product is supplied in a 500 mL liquid format under SKU CCM-SC-0019, with a listed unit price of USD 643.06. For related formulations, visit the stem cell media category or submit requirements through the request quote page for volume pricing, documentation, and supply planning.

• Serum-free expansion of human mesenchymal stromal cells in R&D and process-development settings.
• Evaluation of animal-origin-free medium conditions for hMSC culture workflows where reduced raw-material variability is required.
• CO2 incubator culture using bicarbonate buffering at 37°C and 4–6% CO2.
• Media qualification studies comparing pH, osmolality, endotoxin level, growth performance, and morphology across lots.
• Upstream cell-culture process development, including flask, multilayer vessel, and compatible closed or semi-closed culture systems after site-specific qualification.
• Academic and industrial hMSC model systems requiring a defined, serum-free liquid medium without fetal bovine serum substitute.

Store CCM-SC-0019 at 2–8°C protected from light. Do not freeze unless validated by the receiving laboratory, as freeze-thaw exposure may affect formulation performance. The product ships on blue ice to help maintain refrigerated transit conditions. Before use, visually inspect the bottle for leakage, turbidity, precipitate, or unexpected color change, and equilibrate only the required volume to working temperature using aseptic technique. The stated shelf life is 12 months when the product is stored unopened under recommended conditions. After opening, handle in a qualified biosafety cabinet or equivalent controlled environment, minimize repeated warming cycles, and follow internal hold-time procedures for opened liquid media.

CellCultureMedia supplies CCM-SC-0019 for research and bioprocessing use with quality documentation suitable for supplier qualification, incoming inspection, and lot traceability. Manufacturing and quality release are performed under ISO-aligned controls, with sterility by filtration and specification testing for pH, osmolality, endotoxin, appearance, and other applicable release parameters. A lot-specific Certificate of Analysis can be requested through the quality documentation team. Free worldwide shipping is available where import conditions permit, and procurement teams may request coordinated delivery schedules, multi-bottle orders, and bulk pricing support through CellCultureMedia.