Recombinant Trypsin 10X, Phenol Red-Free

CCM-RG-0047 is a concentrated recombinant trypsin solution for cell digestion and dissociation. The 10X format allows users to prepare working-strength reagent according to internal SOPs, reducing storage volume and improving consistency across repeated passaging operations. The formulation is phenol red-free and includes 1 mM EDTA to support cell detachment by chelating divalent cations that contribute to cell adhesion. Because the enzyme source is recombinant and the product is classified as animal-origin-free, it is suited to workflows that restrict animal-derived raw materials.

This liquid reagent is supplied in a 500 mL size for laboratories and process groups requiring larger-volume cell culture reagents. Key specifications include pH 7.0–7.4, osmolality of 300–380 mOsmol/kg, endotoxin not more than 10 EU/mL, and a stated shelf life of 36 months when stored as directed. It belongs to the cell digestion and dissociation reagent class within cell culture reagents and is intended for controlled use with compatible cell culture systems.

• Routine detachment and passaging of adherent mammalian cells in research and development laboratories.
• Process development studies requiring animal-origin-free cell dissociation chemistry for raw material control.
• Comparability work where phenol red-free reagents are preferred to minimize color interference in downstream analytical readouts.
• Scale-up workflows where a 10X concentrate supports preparation of working solutions matched to batch size and process timing.
• Cell harvest steps prior to counting, viability assessment, subculture, cryopreservation preparation, or analytical sampling.

Users should optimize exposure time, dilution, temperature, and neutralization conditions for each cell type and culture format. Overexposure to proteolytic reagents can affect cell surface proteins or reduce post-detachment recovery, so validated internal procedures should define maximum contact time and quench conditions.

Store CCM-RG-0047 at 2–8 °C and protect containers from avoidable temperature excursions. The product is shipped on blue ice to maintain refrigerated conditions in transit. Do not freeze unless qualified by your internal stability protocol, and minimize repeated warming during use. Handle with aseptic technique after opening, close the container promptly, and use sterile vessels or pipetting systems compatible with your cell culture process. The labeled shelf life is 36 months under recommended storage conditions; always verify the expiration date and lot information before release to production or experimental use.

CellCultureMedia supplies CCM-RG-0047 for professional cell culture, R&D, and bioprocessing environments. Manufacturing and release controls are managed under ISO-aligned quality practices, with lot traceability and specification review available to support supplier qualification. A Certificate of Analysis can be requested through the quality documentation workflow and may include lot-specific values such as pH, osmolality, endotoxin, appearance, and release status according to available records.

The listed 500 mL unit price is USD 2,866.67, with free worldwide shipping for standard qualified orders. Procurement teams planning multi-lot reservations, standing orders, or bulk supply can submit requirements through request a quote for lead time confirmation, documentation needs, and volume pricing review.