Phenol Red-Free Serum-Free hMSC Culture Medium

CCM-SC-0020 is a phenol red-free, serum-free hMSC culture medium supplied as a ready-to-use liquid. The formulation includes D-glucose, L-glutamine, and sodium bicarbonate, and it is manufactured without added serum, fetal bovine serum substitute, or phenol red. This composition is suitable for laboratories that need reduced variability from undefined serum components and simplified visual interpretation where phenol red interference is not desired.

Key specifications include pH 7.0–7.4 and osmolality 280–320 mOsmol/kg, supporting compatibility with standard mammalian cell culture conditions. The product is classified as animal-origin-free and is filter sterilized. Endotoxin is specified at ≤3 EU/mL, and the shelf life is 12 months when stored as directed. For related defined media options, see our stem cell media category or contact CellCultureMedia for lot and volume planning.

• Expansion and maintenance of human mesenchymal stem cells in serum-free research culture systems.
• Phenol red-free culture workflows where optical, colorimetric, fluorescence, or analytical readouts benefit from reduced background interference.
• Process development programs requiring an animal-origin-free medium input with defined pH, osmolality, sterility, and endotoxin specifications.
• Feasibility studies, scale-up evaluation, and comparative medium screening for hMSC manufacturing development.
• Academic, biotech, and bioprocess laboratories standardizing liquid medium supply for 37 °C incubation with 4–6% CO2.

Store CCM-SC-0020 at 2–8 °C protected from light. Do not use beyond the stated 12-month shelf life or if the bottle integrity is compromised. Handle using aseptic technique in a qualified biosafety cabinet, and pre-warm only the volume required for immediate use to reduce repeated temperature cycling. The medium is supplied filter sterile and should not be re-filtered unless required by an internal validated procedure. Shipping is performed on blue ice to maintain refrigerated transit conditions; upon receipt, inspect the package, record temperature indicators if present, and transfer the bottle promptly to refrigerated storage.

CellCultureMedia supplies CCM-SC-0020 for professional research and bioprocess use with procurement-ready traceability. Manufacturing and release are performed under ISO-aligned quality practices, with specification checks covering appearance, sterility by filtration process control, endotoxin limit, pH, osmolality, fill volume, and labeling. A Certificate of Analysis can be requested for applicable lots through our quality documentation page. For standing orders, multi-bottle demand, or site-specific documentation needs, submit requirements through request a quote. The listed 500 mL price is $643.06, and eligible orders include free worldwide shipping with refrigerated blue-ice packaging.