PBS pH 7.2 Without Calcium, Magnesium, Phenol Red

CCM-RG-0007 is a ready-to-use phosphate buffered saline formulation for cell culture-related reagent workflows. It is supplied as a clear liquid balanced salt solution with no added calcium ions, no added magnesium ions, and no phenol red. The absence of divalent cations makes it suitable for washing steps prior to dissociation procedures or downstream handling in which calcium- or magnesium-dependent adhesion, aggregation, or reagent activity needs to be minimized. The absence of phenol red supports workflows where colorimetric interference, background signal, or indicator carryover should be avoided.

The product is manufactured as an animal-origin-free reagent and filter sterilized for use in controlled laboratory processes. Key release specifications include pH 7.1–7.3, with a target pH of 7.2, osmolality of 280–320 mOsmol/kg, and endotoxin not more than 1 EU/mL. The formulation is provided in a 500 mL bottle for benchtop cell culture operations, small-scale process development, assay preparation, and routine buffer exchange. For related formulations and accessory reagents, review the cell culture reagents category or request lot-specific documentation before purchase.

• Rinsing adherent cell monolayers before dissociation, medium change, staining, or sample preparation steps where calcium and magnesium are not required.
• Washing suspension cells, cell pellets, tissue culture inserts, vessels, and process-contact surfaces during laboratory-scale cell handling.
• Preparing cell suspensions, diluting reagents, or equilibrating samples in an isotonic phosphate buffered environment with osmolality specified at 280–320 mOsmol/kg.
• Supporting assays where phenol red is undesirable because of optical readouts, fluorescence background, absorbance monitoring, or downstream analytical sensitivity.
• Use as a general balanced salt solution in mammalian cell culture, academic research, process development, and quality-control sample workflows.
• Routine procurement for laboratories standardizing animal-origin-free ancillary materials with defined sterility, endotoxin, pH, and storage specifications.

Store CCM-RG-0007 at 2–30 °C and protect the bottle from light during warehousing and routine laboratory use. The product ships at ambient temperature, which simplifies receiving and internal distribution for multi-site procurement teams. The stated shelf life is 48 months when the bottle is stored unopened under recommended conditions. Use aseptic technique after opening, keep the container closed when not in use, and avoid repeated exposure to non-controlled environments. Do not use if the solution shows visible particulates, turbidity, container damage, or other signs of compromised integrity.

CellCultureMedia supplies CCM-RG-0007 for research, development, and bioprocessing support under ISO-aligned supplier quality practices. Each lot is controlled against defined specifications for sterility by filtration, pH, osmolality, endotoxin, appearance, and fill configuration. A Certificate of Analysis is available on request, and procurement teams may reference the CCM SKU when aligning purchasing records, inventory systems, and incoming quality checks. For documentation expectations, visit our quality resources. Standard orders include free worldwide shipping, and larger recurring requirements can be coordinated through request a quote for volume pricing, forecasted supply, or multi-location delivery planning.