Lymphocyte Separation Solution, Density 1.077

CCM-SC-0008 is a cell density gradient separation solution formulated at density 1.077 for lymphocyte enrichment and related cell culture reagent workflows. It is supplied as a liquid, sterile-filtered reagent and is classified as animal origin-free, supporting laboratories that need defined materials for cell processing, assay setup, and upstream research operations. The product is intended for controlled separation by centrifugation where a density barrier is used to partition cell populations based on buoyant density.

Key release specifications include pH 7.0 to 7.4, osmolality 270 to 340 mOsmol/kg, endotoxin not more than 0.1 EU/mL, and sterility by sterile filtration. These parameters help procurement and process teams compare lots, qualify incoming material, and align the reagent with internal standard operating procedures. For adjacent reagents used in cell isolation, expansion, and assay preparation, see our stem cell media and reagents category.

• Density gradient separation of lymphocyte-enriched fractions for research cell culture workflows.
• Preparation of cell populations used in immunology, stem cell, and primary cell research programs.
• Upstream sample processing before culture initiation, cell counting, viability assessment, phenotyping, or downstream assay setup.
• Standardized reagent sourcing for laboratories moving from small-batch testing to repeatable process development.
• Use in academic, biotechnology, and biopharma R&D environments that require animal origin-free liquid reagents with defined pH, osmolality, endotoxin, and sterility specifications.

The solution is provided ready to use, reducing preparation variability associated with in-house density media formulation. As with any separation reagent, users should qualify the product with their sample type, centrifugation parameters, rotor format, interface recovery method, and acceptance criteria. For larger campaigns, multi-site supply planning, or standing purchase requirements, submit details through request a quote so CellCultureMedia can confirm availability, pack configuration, and volume pricing.

Store CCM-SC-0008 at 2 to 30 °C protected from light. The product ships at room temperature, which supports simplified international logistics and reduces cold-chain handling complexity. Shelf life is 36 months when stored as recommended and kept in the original closed container. Before use, inspect the bottle for clarity, container integrity, and labeling. Handle using aseptic technique, avoid unnecessary exposure to light during setup, and do not return unused liquid to the original bottle after dispensing. Laboratories should document lot number, opening date, and in-use controls according to their internal quality system.

CellCultureMedia supplies CCM-SC-0008 under ISO-aligned sourcing and release practices appropriate for research and bioprocessing reagent supply. Lot-specific documentation can be requested to support incoming inspection, material qualification, and audit files, including Certificate of Analysis availability for the relevant production lot. The stated specifications cover form, density use class, animal origin-free classification, sterility, endotoxin, osmolality, pH, shelf life, storage, and shipping conditions. Additional documentation requests may be reviewed through our quality and documentation channel. Free worldwide shipping is included for standard orders, and procurement teams may request consolidated shipments for multi-item sourcing.