L-Alanyl-L-Glutamine 200 mM Supplement

L-alanyl-L-glutamine is a soluble dipeptide form of glutamine used to supplement cell culture media where conventional L-glutamine stability can be a process limitation. In aqueous media, free L-glutamine can degrade over time, generating ammonia and reducing nutrient consistency. The dipeptide format provides a more stable glutamine source that is enzymatically utilized by cultured cells, supporting reproducible nutrient availability during routine expansion, assay preparation, and controlled bioprocess development work.

CCM-RG-0038 is supplied as a ready-to-use 200 mM liquid concentrate in a 100 mL format. The formulation is animal origin-free and sterile-filtered for direct addition to compatible media under aseptic technique. Key release specifications include pH 4.7 to 6.0 and osmolality 440 to 550 mOsmol/kg. These parameters help procurement and process teams assess fit with existing media formulations, osmolality targets, and change-control requirements. The product is classified for cell culture reagent use and is suitable for inclusion in documented media preparation workflows.

• Glutamine supplementation of mammalian cell culture media used for CHO, HEK293, hybridoma, Vero, epithelial, fibroblast, and other established research cell lines.
• Replacement of free L-glutamine in media development studies where improved solution stability and reduced ammonia generation are important process considerations.
• Preparation of complete media for cell expansion, transfection support studies, clone screening, productivity evaluation, and analytical assay workflows.
• Use in serum-free, chemically defined, serum-reduced, or serum-containing systems after compatibility confirmation by the user’s laboratory.
• Small-scale process development, pilot preparation, and routine R&D media supplementation where a sterile-filtered, animal origin-free liquid concentrate is preferred.

The recommended working concentration depends on the base medium, cell type, and process duration. Many users evaluate L-alanyl-L-glutamine as a functional substitute for standard glutamine supplementation, then confirm growth, viability, metabolite profiles, and product-quality attributes within their own culture model. For larger bottle counts, standing supply, or site-specific documentation needs, procurement teams may request a quote with the CCM-RG-0038 SKU, target quantity, and destination country.

Store CCM-RG-0038 at 2 to 8 ℃ and protect from light. Maintain the container closed when not in use, and handle the supplement using aseptic technique inside an appropriate clean bench, biosafety cabinet, or controlled preparation area. The product is shipped on blue ice to support temperature-controlled transit. Do not use if the container integrity is compromised, if visible contamination is observed, or if the material has been stored outside validated site procedures. After opening, laboratories should apply their internal hold-time, labeling, and traceability controls for sterile liquid reagents. Avoid repeated warming cycles where possible, and return the bottle promptly to refrigerated, light-protected storage after aliquoting or media preparation.

CellCultureMedia supplies CCM-RG-0038 for research, development, and bioprocessing use in cell culture workflows. Manufacturing and release controls are ISO-aligned and emphasize lot traceability, sterile filtration, and specification-based documentation. A Certificate of Analysis is available on request and can be matched to the delivered lot for purchasing files, incoming inspection, and process records. Available specification data include appearance as a liquid supplement, animal origin-free classification, sterile-filtered status, pH range, osmolality range, storage temperature, and blue-ice shipping condition. Additional quality information can be reviewed through our quality documentation resources. Orders include free worldwide shipping unless a customer-specific freight program or special routing instruction is agreed in advance.