Insulin-Free Neuronal Serum-Free Supplement 50X

This insulin-free neuronal serum-free supplement is designed as a concentrated 50X additive for defined culture systems used with neural, neuronal, and related cell models. The liquid format supports direct dilution into compatible basal media and process media after controlled thawing and aseptic handling. For procurement planning, CCM-RG-0071 is supplied as 10 mL, with a listed unit price of US$805.56, a 24-month shelf life from manufacture when stored as specified, and dry-ice logistics for temperature-controlled transit.

The formulation is intended for workflows where insulin is intentionally excluded from the supplement package. This can support experimental designs evaluating insulin-responsive pathways, media optimization studies, and process comparisons where insulin is supplied separately or omitted. The product is serum-free but is classified as animal-origin, so it should be evaluated against internal sourcing, traceability, and documentation requirements before implementation. Related defined supplements and liquid additives are listed in our cell culture reagents category for side-by-side procurement review.

• Supplementation of serum-free neuronal culture systems during R&D media screening, assay development, and routine maintenance workflows.
• Culture of neural lineage cells where a defined, insulin-free supplement is required to separate the effect of the supplement package from separately controlled insulin inputs.
• Optimization of low-serum or serum-free conditions in academic, biotech, and biopharma laboratories using compatible basal media and aseptic technique.
• Comparative studies of media formulations, feed strategies, or supplement concentrations at small scale before transfer into larger controlled culture formats.
• Support for procurement-standardized reagent panels where liquid format, frozen storage, animal-origin classification, shelf life, and dry-ice shipping requirements must be documented in advance.

Recommended working concentration is typically achieved by diluting the 50X supplement 1:50 into the final medium, unless an internal protocol specifies an alternate concentration. Because final pH, osmolality, and performance depend on the receiving medium and other added components, users should verify the complete formulation against their established culture acceptance criteria.

Store CCM-RG-0071 at -30 to -5 °C and protect from light throughout inventory holding. The source specification lists a 24-month shelf life under these conditions. The product ships on dry ice and should be transferred to qualified frozen storage promptly upon receipt. Thaw only the amount needed for immediate use, mix gently after thawing, and avoid vigorous agitation. Repeated freeze-thaw cycles can introduce variability in supplement performance; aliquoting into sterile, qualified containers may be appropriate when permitted by your site procedure. Use aseptic technique when opening the container or preparing diluted medium, and record lot number, thaw date, operator, and receiving medium for traceable process records.

CellCultureMedia supplies this reagent for research, cell culture development, and bioprocess support applications. Manufacturing and release documentation are managed within ISO-aligned quality practices, with lot-specific information available to support incoming inspection and material qualification. A Certificate of Analysis can be requested through our quality documentation team, including available release attributes and traceability details for the supplied lot.

For purchasing teams, the CCM-* SKU structure simplifies ordering without reliance on source catalog identifiers. Bulk pricing, scheduled shipments, and multi-item reagent consolidation can be requested through request a quote. Free worldwide shipping is available on eligible orders, subject to dry-ice service availability, customs requirements, and destination import rules for animal-origin research materials.