HBSS with Calcium, Magnesium and Bicarbonate

CCM-RG-0011 is Hank’s Balanced Salt Solution supplied as a ready-to-use liquid reagent for cell culture-related processing. The formulation contains 1.0 g/L D-glucose, 0.35 g/L sodium bicarbonate, calcium ions, and magnesium ions, and it is manufactured without phenol red. The absence of phenol red is useful where optical interference, color contribution, or assay background from indicator dye is not desired. As a balanced salt solution, it provides inorganic salts, glucose, and buffering support for washing, rinsing, dilution, and interim cell handling steps outside complete growth medium.

The product is classified as animal-origin-free and filter sterilized. Key release specifications include pH 7.0–7.6, osmolality 260–300 mOsmol/kg, and endotoxin not more than 1 EU/mL. The liquid format reduces preparation variability compared with in-house powder reconstitution and supports consistent use across R&D, process development, and academic laboratory environments. Procurement teams can source this reagent within the broader cell culture reagents portfolio for harmonized purchasing and documentation workflows.

• Washing adherent or suspension cells during medium exchange, subculture preparation, or sample processing when a balanced isotonic salt environment is required.
• Short-term holding and manipulation of mammalian cells during routine culture operations where calcium and magnesium are compatible with the workflow.
• Rinsing culture vessels, cell layers, or harvested cell material before downstream analytical preparation.
• Dilution or transfer steps in cell culture-related reagent workflows requiring HBSS without phenol red.
• Assay preparation where removal of phenol red helps reduce background color contribution in optical measurements.
• Process development studies that require a defined, sterile, animal-origin-free balanced salt solution with documented pH, osmolality, endotoxin, and shelf-life attributes.

Store CCM-RG-0011 at 2–30 °C protected from light. The product ships at ambient temperature and has a stated shelf life of 24 months when stored unopened under recommended conditions. Inspect the bottle before use and do not use if the container is compromised, visibly contaminated, or outside internal acceptance criteria. Use aseptic technique after opening to help maintain sterility, and minimize repeated warming, prolonged light exposure, and unnecessary handling. Because this formulation contains calcium and magnesium, confirm compatibility with dissociation reagents, chelators, and cell-specific protocols before implementation in a validated workflow.

This HBSS is produced under ISO-aligned quality practices for research and bioprocessing supply chains, with filtration-based sterility control and release specifications covering pH, osmolality, endotoxin, format, shelf life, and storage conditions. A Certificate of Analysis is available on request through quality documentation support. CellCultureMedia provides free worldwide shipping for this 500 mL unit and can support consolidated ordering for laboratories standardizing multiple balanced salt solutions, sera, media, and reagents across purchasing locations.