HBSS with Phenol Red, without Calcium and Magnesium

CCM-RG-0012 is a ready-to-use Hank's Balanced Salt Solution for cell culture workflows that require an isotonic salt matrix without divalent calcium or magnesium ions. The formulation contains phenol red for visual pH monitoring, 1.0 g/L D-glucose as an energy source, and 0.35 g/L sodium bicarbonate for buffering in CO2-compatible handling environments. The absence of Ca2+ and Mg2+ is useful when protocols require reduced cell-cell or cell-surface adhesion during washing steps, reagent dilution, or preparation before dissociation procedures. For related buffers, rinses, and liquid handling reagents, see our cell culture reagents category.

The product is supplied as a liquid in a 500 mL bottle and is classified as animal origin-free. Key release specifications include sterile-filtered manufacturing, endotoxin not more than 1 EU/mL, pH 7.0 to 7.4, and osmolality 260 to 300 mOsmol/kg. This specification profile supports procurement teams that need consistent lot documentation for research, assay development, and upstream process support materials. The listed unit price is $47.22 for 500 mL, with quote support available for standing orders, multi-bottle purchases, and laboratory supply consolidation.

• Rinsing adherent mammalian cell monolayers before media exchange, passaging, transfection setup, or analytical sampling when calcium and magnesium are not required by the protocol.
• Dilution or preparation of cell culture reagents where an isotonic, buffered salt solution with phenol red is preferred for visual pH status checks during bench handling.
• Short-duration holding, washing, or transfer of suspension cells in research and bioprocess development workflows, provided the exposure time and temperature are validated by the user.
• Pre-dissociation washing steps before use of separately sourced detachment reagents, where removal of serum proteins and exclusion of Ca2+ and Mg2+ can improve procedural consistency.
• Assay preparation workflows that require a defined balanced salt solution containing D-glucose and sodium bicarbonate but excluding added calcium chloride and magnesium salts.

Store CCM-RG-0012 at 2 to 30 °C and protect the bottle from light to preserve phenol red performance and formulation integrity. The product ships at room temperature and does not require refrigerated freight under the stated shipping condition. Shelf life is 24 months when stored unopened within the recommended temperature range. Use aseptic technique after opening, keep the cap closed when not dispensing, and avoid repeated exposure to non-sterile pipettes, tubing, or reservoirs. Do not use material that shows visible contamination, turbidity, precipitate inconsistent with normal appearance, damaged packaging, or a color shift outside the expected pH indicator range for the intended workflow.

CellCultureMedia supplies CCM-RG-0012 for research, development, and bioprocessing support under ISO-aligned manufacturing and quality control practices. Lot-level documentation can be requested through our quality team, including Certificate of Analysis availability with core release attributes such as sterility, endotoxin, pH, osmolality, shelf life, and appearance where applicable. Procurement managers can use the CCM SKU for ordering, receiving, and inventory control across single-site or multi-site programs.

For project pricing, recurring supply, or larger-volume purchasing, submit requirements through request a quote. Include required pack count, delivery country, target arrival date, documentation needs, and any internal vendor qualification forms. Free worldwide shipping is available for eligible orders, and standard room-temperature dispatch helps simplify logistics compared with cold-chain materials. CellCultureMedia can also support cross-referencing against internal material descriptions when teams are standardizing balanced salt solution supply across R&D, pilot, or process engineering groups.