Exosome Expression Serum-Free Medium, ADCF, Phenol Red-Free

CCM-SC-0031 is a ready-to-use serum-free medium for exosome and extracellular vesicle expression studies in cell culture. The formulation is animal-derived component-free and includes D-glucose as an energy substrate, L-glutamine as a nitrogen and carbon source, and sodium bicarbonate for CO2-buffered culture systems. Phenol red is excluded to reduce optical and analytical interference in downstream workflows such as particle analysis, protein measurement, fluorescence-based assays, and conditioned-medium processing.

The product is supplied as a liquid, sterile-filtered medium in a 500 mL format for research and bioprocess development teams that require lot traceability and specification-based purchasing. Key release specifications include pH 7.0–7.4, osmolality 280–320 mOsmol/kg, endotoxin ≤1 EU/mL, and a 12-month shelf life when stored as directed. Recommended culture conditions are 36–38 °C in a humidified incubator with 5–10% CO2. For related media formats, see our stem cell media category or contact CellCultureMedia for formulation matching.

• Serum-free culture workflows for evaluating exosome and extracellular vesicle output from compatible adherent or suspension cell systems.
• Conditioned-medium generation where animal-origin inputs and serum-associated vesicle background need to be reduced through defined culture conditions.
• Phenol red-free culture steps prior to analytical readouts that are sensitive to colorimetric background, fluorescence interference, or absorbance shift.
• Process development runs requiring controlled pH, osmolality, endotoxin, and sterility specifications across repeat lots.
• R&D screening in academic, biotechnology, and biomanufacturing laboratories using cells adapted to serum-free, animal-derived component-free conditions.

Store CCM-SC-0031 at 2–8 °C protected from light. Do not freeze unless a site-specific stability assessment has been completed, because freezing may affect medium appearance or performance. Handle aseptically in a certified biosafety cabinet, keep containers closed when not in use, and avoid repeated warming cycles. Warm only the required volume to room temperature or the validated process temperature before use. The medium is shipped on blue ice to support refrigerated transit; inspect the bottle on receipt for seal integrity, clarity, and label match against your purchase order.

CellCultureMedia supplies CCM-SC-0031 under ISO-aligned quality practices with specification-driven release checks for sterility, endotoxin, pH, osmolality, appearance, and labeling. A Certificate of Analysis can be provided on request to support incoming QC, supplier qualification, and batch record documentation. Procurement teams may use the request a quote page for volume pricing, standing orders, or multi-site supply planning. Additional information on documentation practices, quality controls, and release expectations is available through our quality resources. Free worldwide shipping is available where temperature-controlled logistics lanes support compliant delivery.