DPBS with Calcium and Magnesium, Phenol Red-Free

CCM-RG-0005 is a phenol red-free Dulbecco’s phosphate buffered saline formulation containing calcium and magnesium ions. It is designed as a balanced salt solution for routine cell culture operations where a defined inorganic salt environment is required without serum, proteins, dyes, or animal-derived components. The presence of Ca2+ and Mg2+ supports procedures where divalent cations are required for cell attachment characteristics, cell-to-cell interactions, or compatibility with downstream reagents. The absence of phenol red reduces color interference in workflows that use optical, fluorescence, or absorbance-based readouts.

This product is supplied as a sterile-filtered liquid in a 500 mL format and is classified as animal origin-free. Key release specifications include pH 7.0–7.4 and osmolality of 270–300 mOsmol/kg, aligning it with common isotonic handling requirements for mammalian cell culture. The reagent is positioned in the balanced salt solution segment of our cell culture reagents portfolio and is suitable for procurement programs that require consistent documentation, lot traceability, and predictable supply. For standing orders, multi-site purchasing, or volume planning, buyers can request a quote referencing CCM-RG-0005.

• Rinsing adherent cell monolayers before dissociation, staining, media exchange, or reagent addition when calcium and magnesium should remain present.
• Short-term cell handling, washing, or dilution steps in research cell culture workflows that require an isotonic phosphate buffered saline system.
• Preparation of cell suspensions, reagent dilutions, and assay wash buffers where phenol red-free composition is preferred to reduce background signal.
• Use in upstream process development, academic core laboratories, quality-control development, and non-GMP bioprocess support environments requiring sterile-filtered liquid reagents.
• Compatibility screening for cell lines and primary cell-derived research models where animal origin-free raw materials are part of sourcing requirements.

Store CCM-RG-0005 at 2–30 °C protected from light. The product ships at room temperature, supporting simplified receiving and inventory workflows without cold-chain handling for standard transit. Do not freeze unless internally validated, as freezing may affect container integrity or salt distribution after thawing. Before use, inspect the bottle for seal integrity, clarity, particulates, and expiration status. Handle aseptically after opening and minimize repeated exposure to non-sterile environments. The stated shelf life is 48 months when the product is stored unopened under recommended conditions. Once opened, laboratories should apply their own in-use dating policy based on aseptic technique, frequency of access, and internal quality requirements.

CellCultureMedia supplies CCM-RG-0005 under ISO-aligned quality practices appropriate for research and bioprocess reagent distribution. Lot-specific documentation can be provided on request, including a Certificate of Analysis when available, to support incoming inspection, batch record attachment, and supplier qualification files. Release attributes include sterile-filtered status, animal origin-free classification, pH range, osmolality range, fill size, storage conditions, shipping conditions, and shelf life. For procurement teams standardizing balanced salt solutions across sites, this specification set supports easier comparison, approval, and reorder control.

Free worldwide shipping is available through CellCultureMedia ordering channels, with final logistics confirmed by destination, quantity, and export requirements. For supplier onboarding, quality questionnaires, or documentation review, visit our quality resources or contact the sales team with the CCM SKU, required quantity, and delivery location. Bulk pricing, scheduled releases, and consolidated shipments can be arranged for laboratories and manufacturing support groups that require recurring supply.