DMEM/F-12 (1:1) Medium with Phenol Red

This DMEM/F-12 (1:1) medium is a liquid, sterile-filtered, animal origin-free base formulation intended for controlled cell culture experiments where users add defined supplements according to their protocol. It contains phenol red for visual pH monitoring and 3.15 g/L D-glucose to support energy metabolism in demanding culture systems. The medium is supplied without L-glutamine and without HEPES, giving process engineers and R&D teams flexibility to select fresh glutamine, stable dipeptide alternatives, or custom buffering strategies.

Key release specifications are set for routine research and development procurement: pH 7.0-7.4, osmolality 270-290 mOsmol/kg, endotoxin ≤1 EU/mL, and sterile-filtered liquid form. The medium is commonly used in protocols incubated at 37°C with 5-10% CO2, including workflows that add 10% FBS or other laboratory-qualified supplements. For related formulations, compare options in the stem cell media category or contact CellCultureMedia for lot, packaging, and volume requirements.

• Maintenance and expansion studies for neuronal, glial, endothelial, and mixed cell culture models in research laboratories.
• Serum-supplemented workflows where the medium is paired with 10% FBS or user-defined supplements validated by the laboratory.
• Protocols requiring a DMEM/F-12 nutrient background without pre-added L-glutamine, enabling fresh addition immediately before use.
• CO2 incubator workflows at 37°C and 5-10% CO2 where phenol red provides a rapid visual indication of pH drift.
• Screening, assay development, and process-comparison studies where pH, osmolality, endotoxin, sterility, and shelf-life specifications must be documented for purchasing and internal quality review.

Store CCM-SC-0007 at 2-8°C protected from light. Do not use the medium if the bottle shows leakage, turbidity, unexpected color shift, or compromised closure integrity. Bring only the required volume to room temperature or 37°C before use, and avoid repeated warming of the full bottle. Because the formulation is supplied without L-glutamine, add glutamine or an approved substitute only when required by the validated protocol. Shelf life is 12 months from manufacture when stored unopened under the recommended conditions. Product ships on blue ice to support cold-chain handling during transit.

CellCultureMedia supplies CCM-SC-0007 for scientific and basic research use with ISO-aligned manufacturing controls, sterile filtration, and lot-level release testing. A Certificate of Analysis is available on request and can include relevant specifications such as pH, osmolality, endotoxin, sterility status, appearance, and expiration dating. Procurement teams can review documentation expectations through our quality information page or submit volume and scheduling details through the request quote form. Free worldwide shipping is available for eligible orders, with bulk pricing and recurring supply programs quoted according to destination, quantity, and lead-time needs.