Dispase Solution 10 mg/mL, 10 mL

CCM-RG-0059 is a 10 mg/mL dispase-type neutral protease solution supplied in a 10 mL pack size for cell culture and sample preparation workflows. The enzyme is commonly used when a milder detachment profile is required compared with broad proteolytic digestion, helping laboratories release adherent cells, separate epithelial sheets, or loosen extracellular matrix structures while supporting downstream viability and morphology assessment. It is suitable for R&D, academic core facilities, and process development teams standardizing enzyme exposure time, working dilution, and rinse procedures across multiple operators.

The reagent is part of CellCultureMedia’s cell culture reagents portfolio and is offered with procurement-ready identification under CCM-RG-0059. The supplied concentration is 10 mg/mL, allowing users to dilute into validated buffers or culture-compatible solutions according to their internal protocols. Because optimal exposure depends on cell type, matrix density, temperature, and vessel format, users should qualify incubation time and neutralization or wash steps before scaling use into routine workflows.

• Gentle detachment of adherent primary cells or established cell lines where preserving surface characteristics and morphology is important for downstream analysis.
• Dissociation of epithelial, endothelial, or organoid-derived cultures from extracellular matrix components in research and bioprocess development settings.
• Preparation of cell sheets, tissue fragments, or clustered cultures prior to passaging, counting, imaging, or analytical characterization.
• Optimization studies comparing enzyme concentration, exposure time, and temperature for standardized cell recovery in multi-user laboratories.
• Use in reagent panels alongside buffers, sera, and other process inputs; request coordinated sourcing through CellCultureMedia quotation support when planning recurring demand.

Store CCM-RG-0059 at -30 to -5 °C and protect the container from light. Maintain frozen storage until use, avoid unnecessary temperature cycling, and thaw only the amount required for the planned procedure whenever possible. After thawing, mix gently without foaming and keep the reagent cold during preparation unless the validated protocol specifies otherwise. Use aseptic handling practices in a biosafety cabinet or appropriate clean work area, and do not return unused aliquots to the original container if sterility risk has been introduced. Laboratories should establish internal hold times, aliquoting practices, and acceptance criteria based on their cell type, workflow, and quality system.

CellCultureMedia supplies CCM-RG-0059 for professional research and bioprocessing procurement with lot traceability and documentation support. Manufacturing and supplier qualification are managed through ISO-aligned quality practices, and a Certificate of Analysis can be requested when placing or confirming an order; available lot documentation may include identity, appearance, concentration, sterility-related, or endotoxin-related information depending on the production record. For specifications, change-control questions, or documentation needs, contact the team through the quality documentation channel. The listed 10 mL item is priced at USD 643.06, with free worldwide shipping where applicable and quotation support for bulk, scheduled, or multi-site purchasing.