Chemically Defined Enzyme-Free Cell Dissociation Medium

This chemically defined cell dissociation medium is a ready-to-use liquid reagent for gentle release of adherent cells from culture vessels without phenol red and without digestive enzyme activity. The animal origin-free formulation is intended for laboratories and process groups that want to reduce undefined raw material inputs while maintaining a controlled dissociation step for routine passaging, cell preparation, and scale-up support. The product is supplied as a 100 mL bottle under SKU CCM-RG-0049, with a listed price of $219.44.

Key release specifications include sterile-filtered liquid format, endotoxin ≤1 EU/mL, pH 7.0–7.4, and osmolality of 300–380 mOsmol/kg. The phenol red-free format is useful where colorimetric interference is not desired in downstream measurements or where users prefer a cleaner visual background. For adjacent buffers, supplements, and dissociation products, review our cell culture reagents category or contact the team for lot availability and documentation.

• Enzyme-free detachment of adherent mammalian cells during routine subculture when users need a chemically defined, animal origin-free reagent.
• Preparation of cells for reseeding, expansion, counting, flow cytometry preparation, imaging workflows, or other research assays where phenol red-free handling is preferred.
• Process development studies that compare dissociation conditions, contact time, recovery, and post-release morphology across defined reagent systems.
• Bioprocessing support for laboratories moving from small culture vessels to multilayer vessels, stirred systems, or closed handling formats, subject to internal qualification.
• Academic, biotechnology, and biopharma R&D settings that require documented sterility, endotoxin, pH, osmolality, shelf-life, and cold-chain parameters for procurement review.

Store CCM-RG-0049 at 2–8 °C and protect the bottle from extended room-temperature exposure when not in use. The product ships on blue ice to maintain a refrigerated logistics profile and has a stated shelf life of 24 months when stored unopened under recommended conditions. Use aseptic technique after opening, close the container promptly, and record open-date tracking according to your site procedure. Do not freeze unless an internal stability assessment has been completed by the user. Before use, equilibrate only the required volume to the temperature specified in the validated cell-handling protocol.

CellCultureMedia supplies this dissociation reagent for research and bioprocessing use with procurement-ready specifications: sterile-filtered format, animal origin-free classification, endotoxin limit of ≤1 EU/mL, pH 7.0–7.4, osmolality 300–380 mOsmol/kg, and 24-month shelf life. Manufacturing and release controls are managed under ISO-aligned quality practices, with documentation support available through our quality resources. A Certificate of Analysis can be provided on request for lot-specific review. For forecasted demand, multi-unit orders, or standing supply arrangements, use the request quote form. Free worldwide shipping is available, with refrigerated shipment on blue ice as the standard condition for this item.