DMSO-Free Cryopreservation Solution

CCM-RG-0089 is designed for laboratories and process groups that need a ready-to-use cryopreservation reagent with a defined, animal origin-free profile. The solution is supplied in liquid form and is manufactured for use within cell culture reagent operations, including cryostorage preparation, cell banking, and recovery workflow standardization. Its DMSO-free specification supports users who wish to remove DMSO from freezing media development, comparative cryopreservation studies, or process materials lists.

Key release specifications include sterile-filtered status, pH 7.0–7.4, and osmolality of 270–400 mOsmol/kg. These parameters make the product suitable for controlled handling alongside common mammalian cell culture media systems, while allowing buyers to document incoming material requirements in procurement and batch records. The product is classified as animal origin-free and is supplied as a 100 mL bottle under CellCultureMedia SKU CCM-RG-0089. For projects requiring repeat purchasing, lot reservation, or multiple-pack supply, customers may submit a request through request a quote for current availability and landed-cost confirmation.

• Cryopreservation of cultured mammalian cells in research, development, and bioprocessing environments where a chemically defined reagent is preferred.
• Preparation of working cell banks and research cell stocks when DMSO exclusion is part of the user-defined process specification.
• Comparability studies evaluating DMSO-free cryopreservation against existing freezing formulations, including post-thaw recovery, attachment, expansion, or viability readouts defined by the laboratory.
• Process development workflows that require animal origin-free ancillary materials to simplify documentation, supplier qualification, and raw material risk assessment.
• Small-scale freezing trials, method development, and scale-up planning before transfer to larger controlled-rate freezing or automated fill workflows.
• Academic, biotech, and biopharma cell culture programs requiring a sterile-filtered cryopreservation reagent with stated pH and osmolality ranges.

Store CCM-RG-0089 at 2–30 °C and protect the bottle from light during storage and routine handling. The stated shelf life is 24 months when stored under recommended conditions in the original container. The product ships on blue ice to support controlled transit conditions; upon receipt, inspect the bottle, label, and closure before placing it into qualified storage. Use aseptic technique when dispensing, and minimize repeated warming, cooling, or extended open-container exposure. Before use, review internal protocols for cell density, cooling rate, cryovial fill volume, and thaw workflow, because final performance depends on the cell type, culture condition, passage history, and user-validated freezing method.

CellCultureMedia supplies CCM-RG-0089 as a sterile-filtered, animal origin-free, DMSO-free cryopreservation reagent for research and bioprocessing supply chains. Manufacturing and release documentation are managed under ISO-aligned quality practices, with product identity, lot traceability, and specification review available for procurement qualification. A Certificate of Analysis can be requested for lot-specific review, and additional quality information is available through the CellCultureMedia quality resource. The 100 mL pack is priced at $319.44, with free worldwide shipping available for eligible orders and destination service lanes. For standing orders, multi-site programs, or larger volume planning, contact CellCultureMedia to confirm lead time, export documentation, shipping mode, and bulk pricing options before purchase order release.