Calcium- and Magnesium-Free PBS pH 7.4

CCM-RG-0008 is a ready-to-use liquid phosphate-buffered saline formulated as a balanced salt solution for cell culture support operations. The product is supplied as 500 mL of PBS with a specified pH range of 7.3 to 7.5 and osmolality of 280 to 320 mOsmol/kg, supporting compatibility with common mammalian cell handling procedures. The formulation excludes phenol red, calcium ions, and magnesium ions, making it appropriate for protocols in which divalent cations may interfere with rinsing steps, cell detachment preparation, reagent dilution, or downstream analytical readouts.

The material is classified as animal-origin-free and filter-sterilized. Endotoxin is controlled at ≤1 EU/mL, supporting use in R&D, process development, and bioprocess support environments that require documented reagent specifications. As part of the CellCultureMedia cell culture reagents portfolio, this PBS is positioned for procurement teams standardizing routine buffer supply across academic, biotech, and biopharma laboratories. The list price is $47.22 per 500 mL unit; buyers planning recurring use, multi-site purchasing, or larger quantities may request a quote for current availability and volume pricing.

• Rinsing adherent cell monolayers before dissociation workflows where calcium- and magnesium-free PBS is specified.
• Washing suspension or adherent cells prior to counting, passaging, medium exchange, or sample preparation.
• Dilution of compatible cell culture reagents, provided the end-user protocol supports phosphate-buffered saline without divalent cations.
• Short handling steps requiring an isotonic balanced salt solution with pH 7.4 nominal conditions.
• Preparation of control blanks, wash fractions, or buffer-only process steps in non-phenol-red workflows.
• General laboratory standardization where animal-origin-free, sterile PBS with defined endotoxin, osmolality, and shelf-life specifications is required.

Store CCM-RG-0008 at 2 to 30 °C protected from light. The product ships at ambient temperature and does not require frozen transport under the stated shipping conditions. Keep the bottle closed when not in use, use aseptic technique during dispensing, and avoid repeated exposure to non-sterile environments. The stated shelf life is 48 months when stored as directed in the unopened container. Do not use material that shows visible contamination, leakage, precipitation outside expected appearance, or a compromised closure. For process-controlled laboratories, record lot number, opening date, storage location, and in-use period according to site procedures.

CCM-RG-0008 is supplied to specification for pH, osmolality, sterility by filtration, endotoxin limit, formulation exclusions, and storage conditions. CellCultureMedia supports procurement and technical review with lot-specific documentation where available, including Certificate of Analysis on request. Our quality approach is designed for ISO-aligned supplier qualification, incoming inspection, and controlled laboratory purchasing; additional information is available through our quality documentation resources. Free worldwide shipping is available, and order support can confirm carton configuration, lead time, and documentation needs before purchase order placement.