Animal-Origin-Free Type IV Collagenase Solution, 1 mg/mL

CCM-RG-0058 is a Type IV collagenase solution formulated at 1 mg/mL for controlled enzymatic dissociation of collagen-containing matrices prior to downstream cell culture operations. The reagent is classified as animal-origin-free, supporting workflows where procurement teams need clear material-origin controls and where process engineers are limiting undefined animal-derived inputs. Type IV collagenase is commonly selected when extracellular matrix digestion is required with comparatively broad activity against collagen structures associated with tissue fragments, organoid-processing steps, or extracellular matrix-coated culture systems.

The product is supplied in a 10 mL bottle for bench-scale R&D, assay development, and small process-development runs. Published pricing is $97.22 per 10 mL unit. Release specifications provided for this item include endotoxin <10 EU/mL, a 24-month shelf life when stored as directed, and dry-ice shipment. For related enzymes, buffers, and dissociation materials, see CellCultureMedia cell culture reagents.

• Enzymatic dissociation of collagen-rich extracellular matrix during preparation of primary cell suspensions for in vitro research culture.
• Processing of adherent culture matrices, scaffold fragments, or extracellular matrix-coated systems where collagen digestion is part of the workflow.
• Small-scale R&D optimization studies comparing enzyme concentration, exposure time, temperature, and mechanical dissociation parameters.
• Bioprocess development activities that require an animal-origin-free collagenase input with documented endotoxin and shelf-life specifications.
• Academic and industrial laboratories standardizing reagent inventories for cell isolation, passaging support, and sample preparation before analytical characterization.

Store CCM-RG-0058 at -30 to -5 °C and protect the bottle from light. The specified shelf life is 24 months under these storage conditions. The product is shipped on dry ice to maintain frozen temperature control during transit; transfer to qualified frozen storage promptly upon receipt. For best consistency, thaw only the volume required for immediate work, keep the reagent cold during setup, and minimize repeated freeze-thaw cycling by preparing single-use aliquots under the laboratory’s validated aseptic procedure. Do not use material that has been stored outside the defined temperature range unless it has been evaluated under the user’s internal quality system.

CellCultureMedia supplies CCM-RG-0058 for research and bioprocessing use with procurement-ready documentation support. Manufacturing and release controls are managed in an ISO-aligned quality framework, with lot traceability and Certificate of Analysis availability on request. The CoA can be used to confirm product identity, concentration, endotoxin specification, shelf life, and shipment condition for receiving inspection and batch-record attachment. For documentation expectations, visit our quality page, or use the request quote form for volume pricing, standing orders, and logistics planning. Free worldwide shipping is available, with dry-ice dispatch used for temperature-controlled delivery.