MSC Dissociation Reagent, Phenol Red-Free

CCM-RG-0051 is a ready-to-use liquid reagent for enzymatic dissociation and passaging of adherent hMSC cultures. The formulation is animal-origin-free and phenol red-free, supporting laboratories that need defined reagent inputs and low background color in microscopy, absorbance-based assays, or media-change observation. It is part of the CellCultureMedia cell culture reagents portfolio for cell detachment, dissociation, and routine culture support.

Key release specifications include sterile filtration, pH 7.2 to 7.8, and osmolality 270 to 340 mOsm/kg H2O. The liquid format helps reduce preparation time compared with dry or reconstitution-based reagents and supports consistent handling across R&D, process development, and academic cell culture labs. The 500 mL pack size is suitable for multi-user facilities, scale-up evaluation, and repeat passaging programs where lot traceability and a defined shelf-life window are important purchasing criteria.

• Passaging and dissociation of adherent human mesenchymal stromal cell cultures in flasks, plates, and other standard culture vessels.
• Phenol red-free workflows where colorimetric interference or visual background needs to be minimized during culture observation or downstream assay preparation.
• Animal-origin-free reagent strategies for process development teams standardizing raw material inputs for hMSC expansion workflows.
• Research-scale comparison of dissociation conditions, exposure time, cell recovery, and post-detachment morphology in defined culture systems.
• Use as a cell culture related reagent in laboratories that require sterile-filtered liquid inputs with documented pH, osmolality, storage, and shelf-life specifications.

Store at 2 to 30 °C protected from light. The product is shipped at ambient temperature, which simplifies receipt planning and reduces cold-chain handling burden for purchasing teams. Shelf life is 36 months when stored as specified and handled using aseptic technique. Before use, inspect the liquid for unusual turbidity, leakage, or container damage, and equilibrate according to the receiving laboratory’s validated cell culture procedure. Avoid repeated unnecessary warming cycles, and close the container promptly after dispensing to maintain reagent integrity.

CellCultureMedia supplies CCM-RG-0051 through ISO-aligned manufacturing and quality workflows intended for reproducible cell culture reagent supply. Lot-level documentation and Certificate of Analysis availability can be discussed with the team through quality documentation support. For planned campaigns, standing orders, or multi-bottle demand, submit requirements through request a quote to confirm current availability, bulk pricing, and shipment scheduling. Standard online pricing for the 500 mL format is $402.78, and free worldwide shipping is available for qualifying orders placed through CellCultureMedia.