M4 hPSC Differentiation Medium, Serum-Free

CCM-SC-0024 is a ready-to-use liquid differentiation medium designed for research and process-development programs using human pluripotent stem cells. The formulation is serum-free and animal-origin-free, supporting defined culture conditions where lot traceability, reproducible inputs, and simplified raw-material qualification are important. It is intended for incubation at 37 °C in a humidified atmosphere containing 4-6% CO2. The medium is supplied in a 500 mL bottle for laboratory-scale method development, protocol transfer, and controlled evaluation across hPSC and hESC differentiation workflows.

The formulation includes D-glucose as an energy source, L-glutamine and alanyl-glutamine for nitrogen supply and improved glutamine availability, HEPES for supplemental buffering, and sodium bicarbonate for CO2-equilibrated pH control. Release specifications include pH 7.0-7.4 and osmolality of 280-320 mOsmol/kg, matching common physiological culture ranges used in pluripotent stem-cell differentiation systems. The product is filter sterilized, with endotoxin specified at ≤1 EU/mL and a stated shelf life of 12 months when stored correctly. For adjacent formulations and scale options, visit stem cell media or request procurement support through the quote desk.

• Directed differentiation studies using hPSC or hESC cultures under serum-free, animal-origin-free conditions.
• R&D screening where a defined liquid medium is required to reduce variability from serum or undefined animal-derived components.
• Process characterization for cell and gene manufacturing research, including evaluation of media inputs, feed timing, and culture-response parameters.
• Protocol standardization across academic, biotechnology, and bioprocess development laboratories using CO2 incubator conditions of 37 °C and 4-6% CO2.
• Small-batch feasibility runs and comparability studies where pH, osmolality, sterility status, endotoxin limit, and shelf-life data are needed for material review.

Procurement teams can use the 500 mL pack size for controlled onboarding before larger purchasing discussions. Process engineers may evaluate the medium as part of a defined raw-material strategy, while R&D teams can use it in parallel comparisons with internal protocols. The medium is not supplied with serum and does not require serum supplementation, which helps simplify documentation and supports more consistent experimental design.

Store CCM-SC-0024 at 2-8 °C and protect the bottle from light. Do not leave the medium at room temperature longer than required for aseptic handling, and return unused material to refrigerated storage promptly after use. The product ships on blue ice to maintain chilled conditions during transit. Before use, inspect the bottle for container integrity, visible particulates, color shift, or evidence of temperature abuse. Mix gently by inversion if required; avoid vigorous shaking that can introduce foam. Use aseptic technique in a certified biosafety cabinet and follow the receiving laboratory’s internal procedures for open-bottle hold time. The labeled shelf life is 12 months when stored under the recommended conditions.

CellCultureMedia supplies CCM-SC-0024 for controlled research and bioprocessing workflows where documentation, consistency, and transparent specifications are purchasing priorities. The medium is manufactured under ISO-aligned quality practices and released against defined physicochemical and microbiological criteria, including filter sterility, endotoxin ≤1 EU/mL, pH 7.0-7.4, and osmolality 280-320 mOsmol/kg. A Certificate of Analysis can be requested for lot review, supplier qualification, and incoming quality checks; additional documentation can be discussed through quality support.

The listed pack is 500 mL with a unit price of USD 2,777.78. Free worldwide shipping is available, with chilled blue-ice transport used for temperature-sensitive delivery. For volume pricing, scheduled replenishment, or project-based sourcing, submit the CCM-SC-0024 SKU, required quantity, destination country, and preferred delivery window. Our team can coordinate quotation, availability, and documentation requirements for institutional purchasing systems.