Phenol Red-Free Cell Dissociation Solution

This cell dissociation solution is a liquid reagent for detaching adherent cells from culture-treated surfaces while maintaining a defined, animal-origin-free formulation profile. The product is phenol red-free and contains 1 mM EDTA, supporting workflows where colorimetric indicator carryover is undesirable or where downstream assays require reduced background from media dyes. It belongs in the cell digestion and dissociation reagent class within cell culture reagents and is supplied in a 500 mL bottle under CCM-RG-0098 for procurement traceability.

Key release specifications include pH 7.0–7.4 and osmolality 270–340 mOsmol/kg, providing compatibility with standard mammalian cell culture handling ranges. The reagent is sterile-filtered and specified with endotoxin at ≤1 EU/mL. These parameters make it suitable for routine passaging, seed-train preparation, assay setup, and scale-down process studies where lot-level documentation and repeatable handling conditions are important. The formulation is animal-origin-free, which may simplify material qualification for laboratories seeking to reduce undefined biological inputs in cell culture operations.

• Routine detachment and subculture of adherent mammalian cell lines in research, development, and bioprocess support laboratories.
• Phenol red-free dissociation steps for applications where optical, fluorescence, or absorbance-based readouts may be affected by indicator dye carryover.
• Preparation of cells for counting, viability assessment, reseeding, transfection setup, and assay plate formatting after monolayer culture.
• Animal-origin-free reagent strategies in media and process development programs that require defined or reduced-risk raw material inputs.
• Use in workflows that benefit from EDTA-assisted dissociation, including reduction of divalent-cation-dependent cell adhesion during controlled detachment.

For larger programs, buyers can coordinate volume forecasts, standing orders, and documentation requirements through the request quote channel. Process engineers evaluating interchangeability should qualify exposure time, neutralization or dilution approach, centrifugation conditions, and post-detachment recovery parameters using their own cell type and vessel format.

Store CCM-RG-0098 at 2–8 °C and handle using aseptic technique after opening. The product is shipped on blue ice to maintain refrigerated transport conditions and has a stated shelf life of 36 months when stored as specified and kept within the original container closure system. Do not freeze unless a site-specific stability assessment has been performed. Before use, equilibrate only the required volume to the intended working temperature, mix gently, and return the remaining bottle to refrigerated storage promptly. Users should avoid repeated prolonged warming cycles and should discard material that shows visible contamination, turbidity, or container integrity concerns.

Manufacturing and release are managed under ISO-aligned quality practices with specification checks for appearance, sterility by filtration process control, pH, osmolality, endotoxin, and shelf-life assignment. A lot-specific Certificate of Analysis is available on request, and additional procurement documentation can be coordinated for institutional vendor files. CellCultureMedia supports technical sourcing for research and bioprocessing customers, including free worldwide shipping where available and quotation support for bulk or recurring demand. Visit quality documentation for information on documentation practices, material traceability, and order support expectations.