Animal Origin-Free Cell Dissociation Reagent with EDTA

CCM-RG-0048 is a liquid dissociation medium designed for controlled removal of adherent cell layers from culture-treated vessels, multilayer systems, and development-scale process equipment. The formulation includes 1 mM EDTA to support cell release by chelating divalent cations involved in cell-to-surface and cell-to-cell adhesion. It is supplied without phenol red, making it suitable for workflows where visual dye components may interfere with downstream readouts, imaging, absorbance measurements, or formulation consistency requirements.

This reagent is classified as animal origin-free and is sterile-filtered for cell culture use. Release specifications include pH 7.0-7.4, osmolality 270-340 mOsmol/kg, endotoxin ≤1 EU/mL, liquid form, and a 24-month shelf life when stored as directed. It is positioned within CellCultureMedia cell culture reagents for R&D laboratories, process development groups, and procurement teams standardizing dissociation materials across multiple adherent culture platforms.

• Routine passaging of adherent mammalian cell cultures where an animal origin-free dissociation reagent is preferred.
• Cell harvest from flasks, plates, roller bottles, multilayer vessels, and other surface-dependent culture systems.
• Phenol red-free workflows supporting microscopy, colorimetric assays, protein analysis, and downstream analytical methods sensitive to indicator dyes.
• Process development studies comparing detachment time, cell recovery, post-harvest viability, and aggregation behavior under defined reagent conditions.
• Scale-up support for bioprocess teams seeking a sterile-filtered dissociation medium with defined pH, osmolality, endotoxin, and shelf-life specifications.

Store CCM-RG-0048 at 2-8 °C and protect the bottle from unnecessary temperature excursions. The product ships on blue ice to maintain refrigerated transit conditions; do not freeze unless an internally validated protocol allows it. Before use, bring only the required volume to the intended working temperature using controlled laboratory practice, and avoid repeated warming of the full bottle. Handle aseptically after opening, keep the closure clean, and record first-use date according to site quality procedures. The stated shelf life is 24 months for unopened material stored under the recommended conditions.

CellCultureMedia supplies CCM-RG-0048 for research and bioprocess cell culture workflows with specification-driven documentation for purchasing and technical review. Manufacturing and release controls are ISO-aligned, and a lot-specific Certificate of Analysis is available on request through our quality documentation team. The CoA can support incoming inspection by confirming key attributes such as sterility status, endotoxin limit, pH range, osmolality range, product format, and expiration dating.

The standard pack size is 500 mL, priced at $402.78 before any customer-specific quotation adjustments, distributor terms, or volume agreements. For standing orders, project stocking, or multi-site procurement, request commercial support through Request a Quote. Free worldwide shipping is available where serviceable and compliant with shipping conditions.