Animal-Origin-Free 10 mM Y-27632 Solution, 1000X

CCM-RG-0086 is an animal-origin-free liquid solution of Y-27632 at 10 mM, supplied as a 1000X stock for addition to cell culture media. Y-27632 is a selective Rho-associated coiled-coil kinase inhibitor widely used in cell culture systems to support cell survival after dissociation, single-cell handling, thaw recovery, and stressful passaging steps. The supplied format reduces in-house weighing and solvent-preparation variability, which can be important for R&D groups standardizing protocols across operators, sites, or automation platforms.

Each unit contains 1 mL of liquid reagent and is specified with a 24-month shelf life when stored as directed. The product classification is animal-origin-free, supporting defined culture workflows and supplier-risk reviews. For procurement planning, the product is listed at USD 319.44 per 1 mL unit. It can be sourced as part of broader cell culture reagents purchasing programs, including media supplements, dissociation reagents, buffers, and supporting process materials. Because this is a concentrated 1000X stock, users should validate the final working concentration, addition point, and exposure duration in their own cell system.

• Short-term supplementation during passaging of sensitive adherent or suspension cultures, particularly after enzymatic or mechanical dissociation.
• Support for single-cell seeding workflows, clonal expansion setup, limiting dilution studies, and automated cell dispensing protocols.
• Use during thawing and early recovery steps where dissociation-associated stress may reduce viable attachment or expansion.
• Defined, animal-origin-free cell culture workflows requiring a ready-to-dilute Y-27632 stock rather than laboratory-prepared powder solutions.
• R&D process development studies comparing media formulations, passaging methods, substrate conditions, or recovery windows.
• Scale-up and tech-transfer work where standardized reagent concentration, unit size, storage conditions, and lot traceability are needed for repeatable execution.

Store CCM-RG-0086 at -30 to -5 °C protected from light. The product ships on dry ice to maintain frozen temperature conditions during transit. Avoid unnecessary freeze-thaw cycles; if repeated access is expected, use validated aseptic aliquoting procedures after receipt and keep aliquots protected from light. Thaw only the quantity needed for the intended run, mix gently, and add to culture media using sterile technique in a controlled workspace. The stated shelf life is 24 months when unopened and stored under the specified conditions. Do not use material that has been exposed to uncontrolled temperatures or visible contamination.

CellCultureMedia supplies CCM-RG-0086 for research, development, and bioprocessing use with procurement documentation appropriate for institutional purchasing. Manufacturing and quality systems are ISO-aligned, with lot traceability maintained for released material. A Certificate of Analysis can be requested through quality documentation support; available lot documents may include appearance, concentration, release status, and applicable sterility or endotoxin information where tested for the specific lot. For pricing confirmation, multi-unit supply, standing orders, or documentation before purchase, submit details through request a quote. Free worldwide shipping is available where applicable, with dry-ice packaging used for this frozen reagent.