Animal-Free iPSC/ESC Cell Dissociation Reagent 1X

CCM-SC-0009 is a liquid cell dissociation reagent classified for pluripotent stem cell use, including iPSC and ESC maintenance workflows. The reagent is supplied at 1X working strength to reduce preparation steps, dilution variability, and operator handling time. Its animal-origin-free and human-origin-free classification supports laboratories that require defined raw material profiles for stem cell culture, clone expansion, assay preparation, and early process development. The reagent is also phenol red-free, which can simplify visual assessment of cell suspensions and reduce background interference in workflows where colorimetric indicators are not desired.

Key release specifications include pH 7.2–7.8 and osmolality of 270–340 mOsmol/kg, both relevant to cell handling during dissociation and reseeding. Sterility is achieved by filtration, and the format is a 500 mL bottle suitable for repeated use under aseptic technique. For related pluripotent stem cell media and reagents, see the stem cell media category. Procurement teams can reference CCM-SC-0009 when standardizing pluripotent stem cell passaging reagents across R&D, process development, and academic core facilities.

• Routine passaging of iPSC and ESC cultures where a defined, animal-origin-free dissociation reagent is required.
• Preparation of single-cell or small-clump suspensions for reseeding, expansion, clone handling, and culture scale-up studies.
• Stem cell culture workflows that require phenol red-free handling reagents to reduce color contribution in downstream assay setup.
• R&D comparability studies evaluating detachment time, cell recovery, morphology after reseeding, and lot-to-lot handling consistency.
• Bioprocess development environments using documented pH, osmolality, shelf-life, and sterility specifications for incoming material control.
• Academic, biotech, and contract research laboratories seeking a ready-to-use 1X reagent that fits standard aseptic cell culture operations.

Store CCM-SC-0009 at 2–30 °C protected from light. The product ships at ambient temperature, supporting simplified receiving and global logistics without frozen handling. The stated shelf life is 36 months when the product is stored as recommended and remains unopened within specification. Before use, inspect the liquid for container integrity and visible contamination, and handle only in an appropriate clean bench or biosafety cabinet using aseptic technique. Avoid unnecessary warming cycles, prolonged light exposure, and repeated cap-off time. For lot reservation, delivery planning, or standing orders, contact procurement support through request a quote.

CellCultureMedia supplies CCM-SC-0009 through ISO-aligned quality controls focused on documented raw material status, in-process control, and final release testing. Lot-specific documentation is available on request, including Certificate of Analysis information covering relevant specifications such as pH, osmolality, appearance, sterility status, shelf life, and storage conditions. If your purchasing specification requires additional release attributes, including endotoxin documentation where available, confirm requirements during quotation. See quality and documentation for our supplier qualification approach. The 500 mL unit is listed at USD 805.56, and eligible orders include free worldwide shipping for qualified institutional customers.