ACK Lysing Buffer, 1X, 100 mL

CCM-RG-0060 is a 1X ACK lysing buffer formulated for rapid erythrocyte lysis while maintaining practical compatibility with downstream cell washing, counting, enrichment, and culture setup steps. The formulation contains 8.3 g/L ammonium chloride, 1.0 g/L potassium bicarbonate, and 37 mg/L EDTA-4Na in an aqueous liquid format. This ammonium chloride chemistry is widely used where nucleated cells must be recovered from mixed cell suspensions that contain a high erythrocyte fraction.

The product is supplied sterile-filtered and classified as animal origin-free, supporting procurement programs that require defined reagent inputs and reduced animal-derived material exposure. Key release specifications include pH 7.0–7.6, osmolality 280–320 mOsmol/kg, endotoxin ≤1 EU/mL, and a 12-month shelf life when stored as directed. The 100 mL pack size is suitable for R&D laboratories, pilot process development, and routine sample preparation workflows. Related sterile liquids and workflow reagents can be reviewed in our cell culture reagents category.

• Removal of erythrocytes from leukocyte-rich suspensions prior to washing, counting, flow cytometry preparation, or culture initiation.
• Preparation of primary mammalian cell suspensions from mixed starting materials where erythrocyte carryover may interfere with cell recovery, assay readout, or visual assessment.
• R&D workflows involving PBMC-associated preparations, spleen-derived cell suspensions, bone marrow-derived cell suspensions, and similar nucleated cell populations.
• Process development work requiring a ready-to-use, 1X lysis buffer with controlled pH, osmolality, sterility, and endotoxin specifications.
• Academic, biotech, and biopharma laboratories standardizing reagent inputs across multiple operators or sites.

ACK lysing buffer is not a complete medium and does not provide nutrients, serum replacement, growth factors, or attachment support. Users should optimize exposure time, cell density, temperature, and quench or wash steps for each cell type, then transfer recovered cells into an appropriate culture medium or assay buffer.

Store CCM-RG-0060 at 2–8 °C protected from light. The product is shipped on blue ice and should be transferred to refrigerated storage promptly after receipt. Maintain aseptic technique when opening and dispensing, and avoid introducing pipettes or accessories that may compromise sterility. Do not use if the container is damaged, if the liquid shows unexpected turbidity or particulate matter, or if the product has exceeded its assigned expiration date. Shelf life is 12 months under recommended storage conditions.

CellCultureMedia supplies CCM-RG-0060 under ISO-aligned quality practices for controlled reagent sourcing, lot traceability, and specification-based release. Available quality attributes include sterile-filtered liquid format, animal origin-free classification, endotoxin limit of ≤1 EU/mL, pH 7.0–7.6, and osmolality 280–320 mOsmol/kg. A Certificate of Analysis can be requested for qualified orders, and additional documentation support is available through our quality team. Procurement teams can use the request quote form for current stock status, bulk pricing, standing orders, and destination-specific shipping confirmation. Free worldwide shipping is available for this catalog item.