0.25% Trypsin Solution Without EDTA, 500 mL

CCM-RG-0046 is a 0.25% trypsin solution formulated without EDTA for enzymatic dissociation of adherent cells from culture plastic or other compatible growth surfaces. The absence of EDTA is useful when calcium- and magnesium-dependent cell surface interactions should be minimized less aggressively than with chelator-containing dissociation reagents, or when downstream protocols call for an EDTA-free detachment step. The product is classified as animal-origin and is supplied as a liquid reagent for cell culture-related use in research, development, and bioprocess support environments.

The formulation contains 2.5 g/L trypsin and phenol red for visual pH indication during handling. Key release specifications include pH 7.2-8.0, osmolality 270-320 mOsmol/kg, and sterile filtration for microbial control. The 500 mL bottle size supports repeated passaging, scale-up experiments, and shared laboratory inventories where a standardized dissociation reagent is preferred. For related buffers, wash solutions, and dissociation products, see our cell-culture reagents category.

• Routine detachment of adherent mammalian cells from flasks, plates, roller bottles, and other compatible culture vessels where trypsin-based dissociation is appropriate.
• EDTA-free passaging workflows in which a chelator-free enzyme step is specified by an internal method, process development protocol, or cell line handling note.
• Preparation of single-cell suspensions for cell counting, reseeding, expansion studies, transfection setup, cryopreservation preparation, or analytical sampling.
• Comparability studies between dissociation conditions, including evaluation of enzyme exposure time, neutralization strategy, post-detachment viability, and aggregate level.
• Procurement programs that require documented pH, osmolality, sterility method, shelf life, storage range, and dry-ice shipping conditions before purchase approval.

Store CCM-RG-0046 at -30 to -5 ℃ protected from light. The stated shelf life is 24 months when the product is stored unopened under the specified frozen conditions. Shipments are arranged on dry ice to maintain the required cold chain in transit. After receipt, transfer bottles promptly to validated frozen storage and avoid repeated freeze-thaw cycles where possible; aliquoting under aseptic conditions may be appropriate for high-use laboratories. Thaw only the quantity needed for the planned work, mix gently after thawing, and use aseptic technique to reduce contamination risk. Do not use if the container integrity is compromised, if the solution shows unexpected turbidity, or if internal acceptance checks fall outside laboratory criteria.

CellCultureMedia supplies CCM-RG-0046 for professional cell culture procurement with specification-focused documentation and lot traceability. Manufacturing and release workflows are managed under ISO-aligned quality practices, with sterile filtration as the specified sterility approach and defined release ranges for pH and osmolality. A Certificate of Analysis can be requested for lot-specific review, and additional documentation support is available through our quality resources.

The standard pack is 500 mL, priced at USD 277.78, with free worldwide shipping available for qualified orders. Procurement teams can request current availability, multi-bottle pricing, documentation, and shipping confirmation through the request quote page. For standing orders or multi-site supply, include target delivery dates, annual volume estimate, and any receiving-site cold-chain requirements so the quotation can be aligned with your purchasing workflow.